Basic Analysis Functions
Many of the analysis functions including the following that are available in the ID7000 software can also be used in Spectral Flow Analysis.
Employs fluorescence compensation (unmixing) algorithm – weighted least squares method (WLSM)
This is a spectral unmixing algorithm based on the least squares method applicable for the detection principle of a spectral analysis-type cell analyzer. The variances in the signal that is detected generally increases as the fluorescence intensity increases. In weighted least squares method, this effect is appropriately handled by suppressing the variances in the calculated results by solving the least squares method with a weighting condition that permits errors due to high fluorescence intensity.
Unmixing Viewer
The unmixing results can be checked in a list. Manual adjustment is also possible, as required.
Autofluorescence separation
Employs Sony’s unique spectral optical technology for traditionally-difficult autofluorescence spectral separation for improved data stability and reliability. In Spectral Flow Analysis, you can find the autofluorescence spectrum using a wizard format just like with the ID7000 software.
Standardization mode
Spectral Flow Analysis also supports the analysis of data acquired in standardization mode in the ID7000 software. Standardization mode standardizes the output of each channel so that the SSC and fluorescence detection sensitivities are the same between multiple instruments.
Virtual filter
The virtual filter function provides simple adjustment of channels, and provides the user flexibility for adding fluorochromes easily as needed. The information for each fluorochrome can be monitored visually in a color palette.
Fluorochromes
Often-used fluorochromes are configured as presets. You can also register user-specific fluorochromes.
Preference settings
You can configure software preference settings for each login user for added convenience.
