Analyzing by Concatenating Multiple Samples (when BL-FlowSOM)

When BL-FlowSOM is performed, learning by concatenating multiple samples is possible. This allows them to be handled as a pseudo-large sample.

When using BL-FlowSOM, select the concatenation result after the samples have been concatenated, then run analysis again.

  1. Transfer the samples to be concatenated to the [Analysis] tab with [Start Analysis] in advance.
  2. Display the dialog for configuring the learning conditions, then click [Concatenate Other Samples].

    The [Sample Selection] dialog appears.

  3. Place check marks in the checkboxes for the samples to concatenate, then click [OK].

    The dialog displays all of the samples on the [Analysis] tab.

    Selected samples that can be concatenated are displayed.

    The following samples are excluded.

    • Sample where a gate selected as a target for running advanced analysis does not exist
    • Sample where there is no fluorochrome selected in the [Fluorochrome] list
  4. Check the message, then click [OK].

    The number of samples to be concatenated is displayed.

  5. Click [OK] and run BL-FlowSOM.

    The result of BL-FlowSOM are displayed on the MST plot when learning using concatenated samples finishes.