Analyzing by Concatenating Multiple Samples (when BL-FlowSOM)
When BL-FlowSOM is performed, learning by concatenating multiple samples is possible. This allows them to be handled as a pseudo-large sample.
When using BL-FlowSOM, select the concatenation result after the samples have been concatenated, then run analysis again.
- Transfer the samples to be concatenated to the [Analysis] tab with [Start Analysis] in advance.
- Display the dialog for configuring the learning conditions, then click [Concatenate Other Samples].
The [Sample Selection] dialog appears.
- Place check marks in the checkboxes for the samples to concatenate, then click [OK].
The dialog displays all of the samples on the [Analysis] tab.
Selected samples that can be concatenated are displayed.
The following samples are excluded.
- Sample where a gate selected as a target for running advanced analysis does not exist
- Sample where there is no fluorochrome selected in the [Fluorochrome] list
- Check the message, then click [OK].
The number of samples to be concatenated is displayed.
- Click [OK] and run BL-FlowSOM.
The result of BL-FlowSOM is displayed on the MST plot when learning using concatenated samples finishes.
- Show the result of other concatenated sample.
The results of the other concatenated samples can be checked on MST plots by opening each sample’s worksheet.
