Analyzing by Learning Samples

BL-FlowSOM is a clustering function to extract a cell population automatically by applying the technology of machine learning.

Set the learning conditions, and analyze by learning samples. With BL-FlowSOM, you can select the star, population pie, and median marker value graph modes.

  1. Click [BL-FlowSOM] in [Clustering] on the [Gate] tab of the ribbon.

    The [BL-FlowSOM] dialog appears.

  2. Set each item.

    1. Select the samples to concatenate when analyzing by concatenating samples.

      For details about the operation, see “Analyzing by Concatenating Multiple Samples (when BL-FlowSOM).”

    2. Select the fluorochromes to use as inputs.
    3. Configure the learning parameters.
    4. Click [OK].

    An empty plot appears, and a busy icon is displayed at the top right during learning. When leaning finishes, an MST plot is displayed.

  3. Select the graph mode.

    Right-click within the plot, and select the graph mode from [Graph Mode] in the context menu.

    You can also select the graph mode in the menu at the top right of the MST plot.

    [Star]: Visualizes the expression levels of multiple markers.

    [Population Pie]: Visualizes the proportions of cell populations.

    [Median Marker Value]: Visualizes the expression level of the marker of one type.

  4. Analyze using the various tools.
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