SFA - Life Sciences Cloud Platform

The [Unmixing] tab of the ribbon has the following buttons.

1. [Unmixing]

   These buttons are used to calculate the fluorescence unmixing matrix and to apply the matrix.

   [Matrix]

   Selects the unmixing matrix.

   [Use common matrix]

   Place a check mark in the checkbox to apply the unmixing matrix to all the samples in the sample group.

   [Calculate Matrix]

   Calculates the spectral unmixing matrix.

   [Apply]

   Selects whether fluorescence unmixing is applied to plots on the worksheet. Clicking this button toggles unmixing on/off. This setting is reflected on the unmixing settings.

   [Unmixing Settings]

   Allows you to adjust the fluorescence unmixing. Clicking this button displays the [Unmixing Settings] dialog. See “[Unmixing Settings] Dialog ([Color Panel] Tab)”.

2. [Spectral Reference]

   These buttons are used to adjust spectral references and register spectral references in the library.

   [Spectral Reference Adjuster]

   Allows you to manually correct the fluorescence unmixing by adjusting the spectral reference of the selected matrix. Clicking this button displays the [Spectral Reference Adjuster] dialog.

   • For details about the operating procedure, see “Adjusting Spectral Unmixing Manually (Spectrum).”

   [Unmixing Viewer]

   You can check the results of spectral unmixing by displaying a list of plots for each fluorochrome combination. Clicking this button displays the [Unmixing Viewer] dialog.

   • For details about the operating procedure, see “Checking Fluorochrome Unmixing Results Summary.”

   [Load Spectral Reference]

   Allows you to load a spectral reference from the spectral reference library into the selected matrix. Clicking this button displays the [Load Spectral Reference] dialog. See “[Load Spectral Reference] Dialog”.

   [Add to Library]

   Adds the spectral reference to the library.

3. [Autofluorescence]

   This button is used to find autofluorescence populations.

   [Autofluorescence Finder]

   Allows you to find the autofluorescence spectrum in acquisition data by searching for the autofluorescence populations.

   Clicking this button displays the [Autofluorescence Finder] dialog.

   • For details about the operating procedure, see “Finding Autofluorescence Populations.”

4. [Fluorochrome]

   This button is used to manage the fluorochrome database.

   [Fluorochrome Database]

   Allows you to edit the fluorochrome database. Clicking this button displays the [Fluorochrome Database] dialog. See “[Fluorochrome Database] Dialog”.